Journal: bioRxiv
Article Title: “Tumor-to-endothelium mitochondrial transfer licenses endothelial cells for CD8 + T cell recognition via mitochondrial neoantigen presentation”
doi: 10.64898/2026.01.30.702567
Figure Lengend Snippet: (A) Contrast-enhanced ultrasound analysis of tumor perfusion. Left, perfusion index quantification. Middle, representative parametric perfusion maps. Right, representative time– intensity curves. (B) Quantification of Evans Blue extravasation (vascular leakage) across treatment groups. (C) Representative immunofluorescence images of tumor vasculature (CD31) and pericyte coverage (NG2) with quantification of NG2 + coverage normalized to CD31 + area. (D) Representative EF5 staining for hypoxia and quantification of EF5 + signal. (E) qPCR analysis of vascular/immune-related transcripts in tumors (Ang2, Cxcl10/IP-10, Cxcl11/I-TAC; fold change vs control). Each dot represents one mouse/tumor. Data are mean ± s.e.m. Statistical significance was assessed using one-way ANOVA with multiple-comparison correction.
Article Snippet: Housekeeping gene Rn18s (Mm03928990_g1), PDL-1 (Mm03048248_m1), Perforin (Mm00812512_m1), Ang1 (Mm01233546_g1), Ang2 (Mm00657574_s1), VECAD/Cdh5 (Mm00486938_m1), Vcam1 (Mm01320970_m1), Cxcl10 (Mm00445235_m1), Cxcl11 (Mm00444662_m1), Cxcl9 (Mm00434946_m1), Vegfa (Mm00437306_m1), Pdgfb (Mm00440677_m1), Irf3 (Mm00516784_m1), CD8a (Mm01182107_g1), Ifnγ (Mm01168134_m1), Il12a (Mm00434169_m1), Il12b (Mm01288989_m1), and Gzmb (Mm00442837_m1), TNF (Mm00443258_m1), PTEN (Mm00477208_m1), Itgax (Mm00498701_m1), Tnfsf10 (Mm01283606_m1), Tnfrsf1a (Mm00441883_g1).
Techniques: Immunofluorescence, Staining, Control, Comparison